4N9F
Crystal structure of the Vif-CBFbeta-CUL5-ElOB-ElOC pentameric complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2013-03-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 103.925, 204.002, 247.866 |
| Unit cell angles | 65.51, 90.28, 90.50 |
Refinement procedure
| Resolution | 49.535 - 3.300 |
| R-factor | 0.2648 |
| Rwork | 0.262 |
| R-free | 0.32380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jgh |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.783 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.970 | 3.820 |
| High resolution limit [Å] | 3.300 | 3.820 | 3.690 |
| Number of reflections | 281473 | ||
| <I/σ(I)> | 15 | ||
| Completeness [%] | 95.9 | 64.9 | 98.6 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.22M potassium sulfate, 18% (w/v) polyethylene glycol (PEG) 3350, 100mM Tris HCl, pH 8.0., VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
