4N9F

Crystal structure of the Vif-CBFbeta-CUL5-ElOB-ElOC pentameric complex

Functional Information from GO Data

Chain	GOid	namespace	contents
0	0000122	biological_process	negative regulation of transcription by RNA polymerase II
0	0000209	biological_process	protein polyubiquitination
0	0003677	molecular_function	DNA binding
0	0003713	molecular_function	transcription coactivator activity
0	0005515	molecular_function	protein binding
0	0005634	cellular_component	nucleus
0	0005654	cellular_component	nucleoplasm
0	0006357	biological_process	regulation of transcription by RNA polymerase II
0	0006366	biological_process	transcription by RNA polymerase II
0	0016020	cellular_component	membrane
0	0016513	cellular_component	core-binding factor complex
0	0043371	biological_process	negative regulation of CD4-positive, alpha-beta T cell differentiation
0	0043378	biological_process	positive regulation of CD8-positive, alpha-beta T cell differentiation
0	0043565	molecular_function	sequence-specific DNA binding
0	0045893	biological_process	positive regulation of DNA-templated transcription
1	0003723	molecular_function	RNA binding
1	0005515	molecular_function	protein binding
1	0016020	cellular_component	membrane
1	0019058	biological_process	viral life cycle
1	0020002	cellular_component	host cell plasma membrane
1	0030430	cellular_component	host cell cytoplasm
1	0044423	cellular_component	virion component
2	0003723	molecular_function	RNA binding
2	0005515	molecular_function	protein binding
2	0016020	cellular_component	membrane
2	0019058	biological_process	viral life cycle
2	0020002	cellular_component	host cell plasma membrane
2	0030430	cellular_component	host cell cytoplasm
2	0044423	cellular_component	virion component
3	0006511	biological_process	ubiquitin-dependent protein catabolic process
3	0031625	molecular_function	ubiquitin protein ligase binding
4	0006368	biological_process	transcription elongation by RNA polymerase II
4	0030891	cellular_component	VCB complex
4	0070449	cellular_component	elongin complex
5	0006511	biological_process	ubiquitin-dependent protein catabolic process
6	0000122	biological_process	negative regulation of transcription by RNA polymerase II
6	0000209	biological_process	protein polyubiquitination
6	0003677	molecular_function	DNA binding
6	0003713	molecular_function	transcription coactivator activity
6	0005515	molecular_function	protein binding
6	0005634	cellular_component	nucleus
6	0005654	cellular_component	nucleoplasm
6	0006357	biological_process	regulation of transcription by RNA polymerase II
6	0006366	biological_process	transcription by RNA polymerase II
6	0016020	cellular_component	membrane
6	0016513	cellular_component	core-binding factor complex
6	0043371	biological_process	negative regulation of CD4-positive, alpha-beta T cell differentiation
6	0043378	biological_process	positive regulation of CD8-positive, alpha-beta T cell differentiation
6	0043565	molecular_function	sequence-specific DNA binding
6	0045893	biological_process	positive regulation of DNA-templated transcription
7	0003723	molecular_function	RNA binding
7	0005515	molecular_function	protein binding
7	0016020	cellular_component	membrane
7	0019058	biological_process	viral life cycle
7	0020002	cellular_component	host cell plasma membrane
7	0030430	cellular_component	host cell cytoplasm
7	0044423	cellular_component	virion component
9	0006511	biological_process	ubiquitin-dependent protein catabolic process
9	0031625	molecular_function	ubiquitin protein ligase binding
a	0000122	biological_process	negative regulation of transcription by RNA polymerase II
a	0000209	biological_process	protein polyubiquitination
a	0003677	molecular_function	DNA binding
a	0003713	molecular_function	transcription coactivator activity
a	0005515	molecular_function	protein binding
a	0005634	cellular_component	nucleus
a	0005654	cellular_component	nucleoplasm
a	0006357	biological_process	regulation of transcription by RNA polymerase II
a	0006366	biological_process	transcription by RNA polymerase II
a	0016020	cellular_component	membrane
a	0016513	cellular_component	core-binding factor complex
a	0043371	biological_process	negative regulation of CD4-positive, alpha-beta T cell differentiation
a	0043378	biological_process	positive regulation of CD8-positive, alpha-beta T cell differentiation
a	0043565	molecular_function	sequence-specific DNA binding
a	0045893	biological_process	positive regulation of DNA-templated transcription
b	0003723	molecular_function	RNA binding
b	0005515	molecular_function	protein binding
b	0016020	cellular_component	membrane
b	0019058	biological_process	viral life cycle
b	0020002	cellular_component	host cell plasma membrane
b	0030430	cellular_component	host cell cytoplasm
b	0044423	cellular_component	virion component
B	0006511	biological_process	ubiquitin-dependent protein catabolic process
c	0000122	biological_process	negative regulation of transcription by RNA polymerase II
c	0000209	biological_process	protein polyubiquitination
c	0003677	molecular_function	DNA binding
c	0003713	molecular_function	transcription coactivator activity
c	0005515	molecular_function	protein binding
c	0005634	cellular_component	nucleus
c	0005654	cellular_component	nucleoplasm
c	0006357	biological_process	regulation of transcription by RNA polymerase II
c	0006366	biological_process	transcription by RNA polymerase II
c	0016020	cellular_component	membrane
c	0016513	cellular_component	core-binding factor complex
c	0043371	biological_process	negative regulation of CD4-positive, alpha-beta T cell differentiation
c	0043378	biological_process	positive regulation of CD8-positive, alpha-beta T cell differentiation
c	0043565	molecular_function	sequence-specific DNA binding
c	0045893	biological_process	positive regulation of DNA-templated transcription
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0031625	molecular_function	ubiquitin protein ligase binding
d	0003723	molecular_function	RNA binding
d	0005515	molecular_function	protein binding
d	0016020	cellular_component	membrane
d	0019058	biological_process	viral life cycle
d	0020002	cellular_component	host cell plasma membrane
d	0030430	cellular_component	host cell cytoplasm
d	0044423	cellular_component	virion component
D	0006368	biological_process	transcription elongation by RNA polymerase II
D	0030891	cellular_component	VCB complex
D	0070449	cellular_component	elongin complex
e	0006368	biological_process	transcription elongation by RNA polymerase II
e	0030891	cellular_component	VCB complex
e	0070449	cellular_component	elongin complex
E	0006511	biological_process	ubiquitin-dependent protein catabolic process
f	0006511	biological_process	ubiquitin-dependent protein catabolic process
f	0031625	molecular_function	ubiquitin protein ligase binding
F	0000122	biological_process	negative regulation of transcription by RNA polymerase II
F	0000209	biological_process	protein polyubiquitination
F	0003677	molecular_function	DNA binding
F	0003713	molecular_function	transcription coactivator activity
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0006357	biological_process	regulation of transcription by RNA polymerase II
F	0006366	biological_process	transcription by RNA polymerase II
F	0016020	cellular_component	membrane
F	0016513	cellular_component	core-binding factor complex
F	0043371	biological_process	negative regulation of CD4-positive, alpha-beta T cell differentiation
F	0043378	biological_process	positive regulation of CD8-positive, alpha-beta T cell differentiation
F	0043565	molecular_function	sequence-specific DNA binding
F	0045893	biological_process	positive regulation of DNA-templated transcription
g	0006368	biological_process	transcription elongation by RNA polymerase II
g	0030891	cellular_component	VCB complex
g	0070449	cellular_component	elongin complex
G	0003723	molecular_function	RNA binding
G	0005515	molecular_function	protein binding
G	0016020	cellular_component	membrane
G	0019058	biological_process	viral life cycle
G	0020002	cellular_component	host cell plasma membrane
G	0030430	cellular_component	host cell cytoplasm
G	0044423	cellular_component	virion component
h	0006511	biological_process	ubiquitin-dependent protein catabolic process
H	0006368	biological_process	transcription elongation by RNA polymerase II
H	0030891	cellular_component	VCB complex
H	0070449	cellular_component	elongin complex
i	0000122	biological_process	negative regulation of transcription by RNA polymerase II
i	0000209	biological_process	protein polyubiquitination
i	0003677	molecular_function	DNA binding
i	0003713	molecular_function	transcription coactivator activity
i	0005515	molecular_function	protein binding
i	0005634	cellular_component	nucleus
i	0005654	cellular_component	nucleoplasm
i	0006357	biological_process	regulation of transcription by RNA polymerase II
i	0006366	biological_process	transcription by RNA polymerase II
i	0016020	cellular_component	membrane
i	0016513	cellular_component	core-binding factor complex
i	0043371	biological_process	negative regulation of CD4-positive, alpha-beta T cell differentiation
i	0043378	biological_process	positive regulation of CD8-positive, alpha-beta T cell differentiation
i	0043565	molecular_function	sequence-specific DNA binding
i	0045893	biological_process	positive regulation of DNA-templated transcription
I	0006511	biological_process	ubiquitin-dependent protein catabolic process
I	0031625	molecular_function	ubiquitin protein ligase binding
j	0003723	molecular_function	RNA binding
j	0005515	molecular_function	protein binding
j	0016020	cellular_component	membrane
j	0019058	biological_process	viral life cycle
j	0020002	cellular_component	host cell plasma membrane
j	0030430	cellular_component	host cell cytoplasm
j	0044423	cellular_component	virion component
J	0006368	biological_process	transcription elongation by RNA polymerase II
J	0030891	cellular_component	VCB complex
J	0070449	cellular_component	elongin complex
k	0000122	biological_process	negative regulation of transcription by RNA polymerase II
k	0000209	biological_process	protein polyubiquitination
k	0003677	molecular_function	DNA binding
k	0003713	molecular_function	transcription coactivator activity
k	0005515	molecular_function	protein binding
k	0005634	cellular_component	nucleus
k	0005654	cellular_component	nucleoplasm
k	0006357	biological_process	regulation of transcription by RNA polymerase II
k	0006366	biological_process	transcription by RNA polymerase II
k	0016020	cellular_component	membrane
k	0016513	cellular_component	core-binding factor complex
k	0043371	biological_process	negative regulation of CD4-positive, alpha-beta T cell differentiation
k	0043378	biological_process	positive regulation of CD8-positive, alpha-beta T cell differentiation
k	0043565	molecular_function	sequence-specific DNA binding
k	0045893	biological_process	positive regulation of DNA-templated transcription
K	0006511	biological_process	ubiquitin-dependent protein catabolic process
l	0006511	biological_process	ubiquitin-dependent protein catabolic process
l	0031625	molecular_function	ubiquitin protein ligase binding
L	0000122	biological_process	negative regulation of transcription by RNA polymerase II
L	0000209	biological_process	protein polyubiquitination
L	0003677	molecular_function	DNA binding
L	0003713	molecular_function	transcription coactivator activity
L	0005515	molecular_function	protein binding
L	0005634	cellular_component	nucleus
L	0005654	cellular_component	nucleoplasm
L	0006357	biological_process	regulation of transcription by RNA polymerase II
L	0006366	biological_process	transcription by RNA polymerase II
L	0016020	cellular_component	membrane
L	0016513	cellular_component	core-binding factor complex
L	0043371	biological_process	negative regulation of CD4-positive, alpha-beta T cell differentiation
L	0043378	biological_process	positive regulation of CD8-positive, alpha-beta T cell differentiation
L	0043565	molecular_function	sequence-specific DNA binding
L	0045893	biological_process	positive regulation of DNA-templated transcription
m	0006368	biological_process	transcription elongation by RNA polymerase II
m	0030891	cellular_component	VCB complex
m	0070449	cellular_component	elongin complex
M	0003723	molecular_function	RNA binding
M	0005515	molecular_function	protein binding
M	0016020	cellular_component	membrane
M	0019058	biological_process	viral life cycle
M	0020002	cellular_component	host cell plasma membrane
M	0030430	cellular_component	host cell cytoplasm
M	0044423	cellular_component	virion component
n	0006511	biological_process	ubiquitin-dependent protein catabolic process
N	0000122	biological_process	negative regulation of transcription by RNA polymerase II
N	0000209	biological_process	protein polyubiquitination
N	0003677	molecular_function	DNA binding
N	0003713	molecular_function	transcription coactivator activity
N	0005515	molecular_function	protein binding
N	0005634	cellular_component	nucleus
N	0005654	cellular_component	nucleoplasm
N	0006357	biological_process	regulation of transcription by RNA polymerase II
N	0006366	biological_process	transcription by RNA polymerase II
N	0016020	cellular_component	membrane
N	0016513	cellular_component	core-binding factor complex
N	0043371	biological_process	negative regulation of CD4-positive, alpha-beta T cell differentiation
N	0043378	biological_process	positive regulation of CD8-positive, alpha-beta T cell differentiation
N	0043565	molecular_function	sequence-specific DNA binding
N	0045893	biological_process	positive regulation of DNA-templated transcription
o	0000122	biological_process	negative regulation of transcription by RNA polymerase II
o	0000209	biological_process	protein polyubiquitination
o	0003677	molecular_function	DNA binding
o	0003713	molecular_function	transcription coactivator activity
o	0005515	molecular_function	protein binding
o	0005634	cellular_component	nucleus
o	0005654	cellular_component	nucleoplasm
o	0006357	biological_process	regulation of transcription by RNA polymerase II
o	0006366	biological_process	transcription by RNA polymerase II
o	0016020	cellular_component	membrane
o	0016513	cellular_component	core-binding factor complex
o	0043371	biological_process	negative regulation of CD4-positive, alpha-beta T cell differentiation
o	0043378	biological_process	positive regulation of CD8-positive, alpha-beta T cell differentiation
o	0043565	molecular_function	sequence-specific DNA binding
o	0045893	biological_process	positive regulation of DNA-templated transcription
O	0006511	biological_process	ubiquitin-dependent protein catabolic process
O	0031625	molecular_function	ubiquitin protein ligase binding
p	0003723	molecular_function	RNA binding
p	0005515	molecular_function	protein binding
p	0016020	cellular_component	membrane
p	0019058	biological_process	viral life cycle
p	0020002	cellular_component	host cell plasma membrane
p	0030430	cellular_component	host cell cytoplasm
p	0044423	cellular_component	virion component
P	0006368	biological_process	transcription elongation by RNA polymerase II
P	0030891	cellular_component	VCB complex
P	0070449	cellular_component	elongin complex
q	0003723	molecular_function	RNA binding
q	0005515	molecular_function	protein binding
q	0016020	cellular_component	membrane
q	0019058	biological_process	viral life cycle
q	0020002	cellular_component	host cell plasma membrane
q	0030430	cellular_component	host cell cytoplasm
q	0044423	cellular_component	virion component
Q	0006511	biological_process	ubiquitin-dependent protein catabolic process
r	0006511	biological_process	ubiquitin-dependent protein catabolic process
r	0031625	molecular_function	ubiquitin protein ligase binding
R	0000122	biological_process	negative regulation of transcription by RNA polymerase II
R	0000209	biological_process	protein polyubiquitination
R	0003677	molecular_function	DNA binding
R	0003713	molecular_function	transcription coactivator activity
R	0005515	molecular_function	protein binding
R	0005634	cellular_component	nucleus
R	0005654	cellular_component	nucleoplasm
R	0006357	biological_process	regulation of transcription by RNA polymerase II
R	0006366	biological_process	transcription by RNA polymerase II
R	0016020	cellular_component	membrane
R	0016513	cellular_component	core-binding factor complex
R	0043371	biological_process	negative regulation of CD4-positive, alpha-beta T cell differentiation
R	0043378	biological_process	positive regulation of CD8-positive, alpha-beta T cell differentiation
R	0043565	molecular_function	sequence-specific DNA binding
R	0045893	biological_process	positive regulation of DNA-templated transcription
s	0006368	biological_process	transcription elongation by RNA polymerase II
s	0030891	cellular_component	VCB complex
s	0070449	cellular_component	elongin complex
S	0003723	molecular_function	RNA binding
S	0005515	molecular_function	protein binding
S	0016020	cellular_component	membrane
S	0019058	biological_process	viral life cycle
S	0020002	cellular_component	host cell plasma membrane
S	0030430	cellular_component	host cell cytoplasm
S	0044423	cellular_component	virion component
t	0006511	biological_process	ubiquitin-dependent protein catabolic process
T	0006511	biological_process	ubiquitin-dependent protein catabolic process
u	0000122	biological_process	negative regulation of transcription by RNA polymerase II
u	0000209	biological_process	protein polyubiquitination
u	0003677	molecular_function	DNA binding
u	0003713	molecular_function	transcription coactivator activity
u	0005515	molecular_function	protein binding
u	0005634	cellular_component	nucleus
u	0005654	cellular_component	nucleoplasm
u	0006357	biological_process	regulation of transcription by RNA polymerase II
u	0006366	biological_process	transcription by RNA polymerase II
u	0016020	cellular_component	membrane
u	0016513	cellular_component	core-binding factor complex
u	0043371	biological_process	negative regulation of CD4-positive, alpha-beta T cell differentiation
u	0043378	biological_process	positive regulation of CD8-positive, alpha-beta T cell differentiation
u	0043565	molecular_function	sequence-specific DNA binding
u	0045893	biological_process	positive regulation of DNA-templated transcription
U	0006511	biological_process	ubiquitin-dependent protein catabolic process
U	0031625	molecular_function	ubiquitin protein ligase binding
v	0003723	molecular_function	RNA binding
v	0005515	molecular_function	protein binding
v	0016020	cellular_component	membrane
v	0019058	biological_process	viral life cycle
v	0020002	cellular_component	host cell plasma membrane
v	0030430	cellular_component	host cell cytoplasm
v	0044423	cellular_component	virion component
V	0006511	biological_process	ubiquitin-dependent protein catabolic process
V	0031625	molecular_function	ubiquitin protein ligase binding
w	0006511	biological_process	ubiquitin-dependent protein catabolic process
w	0031625	molecular_function	ubiquitin protein ligase binding
W	0006368	biological_process	transcription elongation by RNA polymerase II
W	0030891	cellular_component	VCB complex
W	0070449	cellular_component	elongin complex
x	0006511	biological_process	ubiquitin-dependent protein catabolic process
x	0031625	molecular_function	ubiquitin protein ligase binding
X	0006368	biological_process	transcription elongation by RNA polymerase II
X	0030891	cellular_component	VCB complex
X	0070449	cellular_component	elongin complex
y	0006368	biological_process	transcription elongation by RNA polymerase II
y	0030891	cellular_component	VCB complex
y	0070449	cellular_component	elongin complex
Y	0006511	biological_process	ubiquitin-dependent protein catabolic process
z	0006511	biological_process	ubiquitin-dependent protein catabolic process
Z	0006511	biological_process	ubiquitin-dependent protein catabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN b 201

Chain	Residue
b	HIS108
b	CYS114
b	CYS133
b	GLU134
b	HIS139

---

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN G 201

Chain	Residue
G	HIS139
G	HIS108
G	CYS114
G	CYS133
G	GLU134

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN M 201

Chain	Residue
M	HIS108
M	CYS114
M	CYS133
M	HIS139

---

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN S 201

Chain	Residue
S	HIS108
S	CYS114
S	CYS133
S	GLU134
S	HIS139

---

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN d 201

Chain	Residue
d	HIS108
d	CYS114
d	CYS133
d	HIS139

---

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN j 201

Chain	Residue
j	HIS108
j	CYS114
j	CYS133
j	GLU134
j	HIS139

---

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN p 201

Chain	Residue
p	HIS108
p	CYS114
p	CYS133
p	HIS139

---

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN v 201

Chain	Residue
v	HIS108
v	CYS114
v	CYS133
v	HIS139

---

site_id	AC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN 1 201

Chain	Residue
1	HIS108
1	CYS114
1	CYS133
1	GLU134
1	HIS139

---

site_id	BC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN 7 201

Chain	Residue
7	HIS108
7	CYS114
7	CYS133
7	GLU134
7	HIS139

---

site_id	BC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN q 201

Chain	Residue
q	HIS108
q	CYS114
q	CYS133
q	HIS139

---

site_id	BC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN 2 201

Chain	Residue
2	HIS108
2	CYS114
2	CYS133
2	HIS139

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	12
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	715
Details	Domain: {"description":"Ubiquitin-like","evidences":[{"source":"PROSITE-ProRule","id":"PRU00214","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	11
Details	Modified residue: {"description":"N-acetylmethionine","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"MAR-2009","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Waridel P.","Quadroni M."]}},{"source":"PubMed","id":"19413330","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"22814378","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	10
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"UniProtKB","id":"P62869","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	48
Details	Binding site: {"evidences":[{"source":"PubMed","id":"24402281","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"37640699","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4N9F","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FVI","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---