4N48
Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 Protein in complex with capped RNA fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-13 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.918410 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.209, 60.038, 87.045 |
| Unit cell angles | 90.23, 97.83, 116.25 |
Refinement procedure
| Resolution | 30.334 - 2.704 |
| R-factor | 0.185 |
| Rwork | 0.182 |
| R-free | 0.24150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n49 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.791 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.334 | 2.860 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 24703 | |
| Completeness [%] | 93.9 | 94.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 291 | 30% PEG 3350, 100 mM Bis-Tris [pH 6.5], and 100 mM NaBr, VAPOR DIFFUSION, temperature 291K |
