4LL1
The structure of the TRX and TXNIP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2009-07-22 |
| Wavelength(s) | 1.100 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.135, 64.022, 88.295 |
| Unit cell angles | 90.00, 91.28, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.22399 |
| Rwork | 0.220 |
| R-free | 0.28806 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Partially refined SeMet-substituted TRX-TXNIP complex structure |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.594 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 58935 | |
| Completeness [%] | 96.6 | 96.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294.15 | 0.16M tri-sodium citrate, 16% PEG3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 294.15K |
