4LGY
Importance of Hydrophobic Cavities in Allosteric Regulation of Formylglycinamide Synthetase: Insight from Xenon Trapping and Statistical Coupling Analysis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Detector technology | CCD |
| Collection date | 2013-02-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97372 |
| Spacegroup name | P 65 |
| Unit cell lengths | 146.329, 146.329, 140.841 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.500 - 1.480 |
| R-factor | 0.14204 |
| Rwork | 0.142 |
| R-free | 0.16623 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t3t |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.855 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.500 | |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.067 | |
| Number of reflections | 274143 | |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 96.5 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 2M ammonium sulphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP |
