4LG8

Crystal structure of PRPF19 WD40 repeats

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-03-04
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	83.055, 83.055, 75.533
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.890
R-factor	0.1581
Rwork	0.156
R-free	0.19680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ow8 2ymu 2co0
RMSD bond length	0.016
RMSD bond angle	1.458
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.920
High resolution limit [Å]	1.890	5.130	1.890
Rmerge	0.088	0.052	0.986
Number of reflections	24511
<I/σ(I)>	10.6
Completeness [%]	100.0	100	100
Redundancy	10.3	9.6	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	20% PEG-3350, 0.2 M disodium tartrate, vapor diffusion, temperature 291K