4JUO
A low-resolution three-gate structure of topoisomerase IV from Streptococcus pneumoniae in space group H32
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97630 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 213.580, 213.580, 211.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.442 - 6.530 |
| R-factor | 0.2498 |
| Rwork | 0.247 |
| R-free | 0.29820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rae |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.491 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.400 | 6.710 |
| High resolution limit [Å] | 6.530 | 6.530 |
| Rmerge | 0.060 | 0.410 |
| Number of reflections | 3701 | |
| <I/σ(I)> | 6.84 | 1.88 |
| Completeness [%] | 99.2 | 99.66 |
| Redundancy | 9.66 | 10.93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 3-4% isopropanol, 50 mM Na cacodylate, optimised mixture of salts, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
