4IOX

The structure of the herpes simplex virus DNA-packaging motor pUL15 C-terminal nuclease domain provides insights into cleavage of concatemeric viral genome precursors

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-03-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97945
Spacegroup name	P 43 21 2
Unit cell lengths	96.944, 96.944, 194.034
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.799 - 2.458
R-factor	0.205
Rwork	0.203
R-free	0.24680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3n4p
RMSD bond length	0.010
RMSD bond angle	1.350
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	3.210	2.500
High resolution limit [Å]	2.458	3.100	2.458
Rmerge	0.086	0.140	0.480
Number of reflections	34365
<I/σ(I)>	42.92	21.6	2.6
Completeness [%]	99.6	100	100
Redundancy	12.2	12.1	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	1 M ammonium citrate, 0.1 M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K