4IJE
Crystal structure of the Zaire ebolavirus VP35 interferon inhibitory domain R312A/K319A/R322A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792075 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 81.012, 81.012, 344.410 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.096 - 1.899 |
R-factor | 0.195 |
Rwork | 0.193 |
R-free | 0.22830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fke |
RMSD bond length | 0.019 |
RMSD bond angle | 0.880 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
High resolution limit [Å] | 1.899 | 5.160 | 1.899 |
Rmerge | 0.130 | 0.058 | |
Number of reflections | 54187 | ||
<I/σ(I)> | 9.3 | ||
Completeness [%] | 99.8 | 98.8 | 99.5 |
Redundancy | 9.1 | 9.3 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298.15 | 2.2 M sodium potassium phosphate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K |