4HW5
Crystal Structure of the Human C3a anaphylatoxin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 63 |
Unit cell lengths | 63.880, 63.880, 105.110 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.651 - 2.250 |
Rwork | 0.244 |
R-free | 0.24270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a73 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.034 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.700 |
High resolution limit [Å] | 2.250 |
<I/σ(I)> | 20.6 |
Completeness [%] | 99.8 |
Redundancy | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 2-2.2 M ammonium sulfate with the pH ranging from 6 to 9 , VAPOR DIFFUSION, SITTING DROP, temperature 292K |