4HOO
Crystal structure of human JMJD2D/KDM4D apoenzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Detector technology | CCD |
| Collection date | 2010-10-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 32 |
| Unit cell lengths | 73.119, 73.119, 136.028 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.930 - 2.495 |
| R-factor | 0.2092 |
| Rwork | 0.207 |
| R-free | 0.24610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hon |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.495 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 18.000 | 18.000 | 2.570 |
| High resolution limit [Å] | 2.495 | 5.670 | 2.495 |
| Rmerge | 0.078 | 0.037 | 0.514 |
| Number of reflections | 25850 | ||
| <I/σ(I)> | 15.5 | ||
| Completeness [%] | 91.5 | 100 | 87 |
| Redundancy | 5.3 | 6.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277.15 | 0.2M calcium acetate, 0.1M HEPES pH 7.5, 10% PEG 8000, vapor diffusion, hanging drop, temperature 277.15K |
