4H3Z

Crystal structure of a symmetric dimer of a tRNA (guanine-(N(1)-)-methyltransferase from Burkholderia phymatum bound to S-adenosyl homocystein in both half-sites

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-28
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	58.850, 188.090, 127.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.200 - 2.150
R-factor	0.1886
Rwork	0.186
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4h3y
RMSD bond length	0.011
RMSD bond angle	1.467
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		2.210
High resolution limit [Å]	2.150	9.620	2.150
Rmerge	0.073	0.023	0.484
Number of reflections	38958	494	2845
<I/σ(I)>	18.35	59.31	4.7
Completeness [%]	99.8	97.6	99.8
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	289	BuphA.00054.a.A1 PS01368 at 35 mg/mL with 3 mM SAH against CSHT screen condition E10, 0.2 M NaCl, 0.1 M NaOAc pH 4.6, 30% MPD, crystal tracking ID 236894e10, unique puck ID zeh2-4, VAPOR DIFFUSION, SITTING DROP, temperature 289K