4GRW
Structure of a complex of human IL-23 with 3 Nanobodies (Llama vHHs)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-07 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 102.270, 135.140, 139.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.830 - 2.550 |
R-factor | 0.184 |
Rwork | 0.183 |
R-free | 0.21800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3d85 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.210 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.9.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.610 |
High resolution limit [Å] | 2.540 | 2.550 |
Rmerge | 0.082 | 0.601 |
Number of reflections | 63755 | |
<I/σ(I)> | 13.5 | 2.65 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 4 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.9 | 277 | 16.5 % PEG 20K and 0.1M MES, pH 5.9, VAPOR DIFFUSION, SITTING DROP, temperature 277K |