4GNK
Crystal structure of Galphaq in complex with full-length human PLCbeta3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2011-03-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.13 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 91.423, 188.845, 293.857 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.990 - 4.000 |
| R-factor | 0.21588 |
| Rwork | 0.214 |
| R-free | 0.25468 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3OHM |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.858 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 4.070 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.140 | 0.329 |
| Number of reflections | 34695 | |
| <I/σ(I)> | 8.8 | 1.9 |
| Completeness [%] | 76.7 | 36.7 |
| Redundancy | 3.3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.75 | 277.15 | 100 mM MES pH 6.75, 100-200 mM NaCl, and 11-12% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.75 | 277.15 | 100 mM MES pH 6.75, 100-200 mM NaCl, and 11-12% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
