4GCW
Crystal structure of RNase Z in complex with precursor tRNA(Thr)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9800 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.080, 42.550, 110.890 |
| Unit cell angles | 90.00, 127.18, 90.00 |
Refinement procedure
| Resolution | 43.300 - 3.000 |
| R-factor | 0.2284 |
| Rwork | 0.227 |
| R-free | 0.25640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fk6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS (PACKAGE) |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.310 | 3.160 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Number of reflections | 13408 | |
| <I/σ(I)> | 10.71 | 2.06 |
| Completeness [%] | 97.9 | 94.6 |
| Redundancy | 6.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 28% PEG MME 2K, 0.1 M Na-MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
