4FLC
Structural and Biochemical Characterization of Human Adenylosuccinate Lyase (ADSL) and the R303C ADSL Deficiency Associated Mutation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9789 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.799, 105.562, 217.221 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.470 - 2.600 |
R-factor | 0.2339 |
Rwork | 0.230 |
R-free | 0.29840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2j91 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.009 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.370 | |
Number of reflections | 58820 | |
<I/σ(I)> | 4.8 | |
Completeness [%] | 95.4 | 97.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1 M Tris pH 8.5, 20% PEG 8000, 12.5 mM spermine tetrahydrochloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K |