4FDC
Crystal structure of the E493V mutant of human apoptosis inducing factor (AIF)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-01-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.400, 62.700, 101.922 |
| Unit cell angles | 90.00, 118.57, 90.00 |
Refinement procedure
| Resolution | 39.500 - 2.400 |
| R-factor | 0.25548 |
| Rwork | 0.253 |
| R-free | 0.29678 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m6i |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.185 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.500 | 2.462 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.450 | 0.530 |
| Number of reflections | 20005 | |
| <I/σ(I)> | 11.3 | 3.1 |
| Completeness [%] | 94.2 | 88.7 |
| Redundancy | 6.8 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under oil | 8.5 | 298 | 30% PEG 1500, 0.1 M TrisHCl pH 8.5, 4% acetone, microbatch under oil, temperature 298K |
