4FBK

Crystal structure of a covalently fused Nbs1-Mre11 complex with one manganese ion per active site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8726
Spacegroup name	P 21 21 21
Unit cell lengths	60.261, 78.872, 222.678
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.884 - 2.379
R-factor	0.2232
Rwork	0.222
R-free	0.24170
RMSD bond length	0.012
RMSD bond angle	1.777
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall
Low resolution limit [Å]	47.884
High resolution limit [Å]	2.379
Number of reflections	43368
Completeness [%]	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 MM Tri Na Citrate pH 5.0, 200 mM ammonium sulfate, 13% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K