4F7Z

Conformational dynamics of exchange protein directly activated by cAMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-09
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 21
Unit cell lengths	69.720, 95.350, 181.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.460 - 2.600
R-factor	0.222
Rwork	0.222
R-free	0.28200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2byv
RMSD bond length	0.005
RMSD bond angle	0.900
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.460	2.660
High resolution limit [Å]	2.600	2.600
Rmerge	0.047	0.590
Number of reflections	37453
<I/σ(I)>	34	1.9
Completeness [%]	98.5	100
Redundancy	3.9	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	100 mM Bis-Tris propane, pH 7.5, 200 mM sodium chloride, 1.3 M ammonium sulfate, 6% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K