4F0A
Crystal structure of XWnt8 in complex with the cysteine-rich domain of Frizzled 8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-01-08 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.03325 |
| Spacegroup name | P 41 |
| Unit cell lengths | 110.070, 110.070, 82.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.640 - 3.250 |
| R-factor | 0.2051 |
| Rwork | 0.203 |
| R-free | 0.23450 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.880 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.640 | 3.300 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Number of reflections | 29898 | |
| <I/σ(I)> | 12.4 | 2 |
| Completeness [%] | 97.7 | 98.7 |
| Redundancy | 2.3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 4-10 % (w/v) PEG 400 15-25 mM Zinc acetate 100 mM Sodium acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
