4EM9
Human PPAR gamma in complex with nonanoic acids
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-07 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.46 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 92.731, 62.255, 118.649 |
Unit cell angles | 90.00, 101.94, 90.00 |
Refinement procedure
Resolution | 32.800 - 2.100 |
R-factor | 0.2065 |
Rwork | 0.205 |
R-free | 0.23510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zeo |
RMSD bond length | 0.005 |
RMSD bond angle | 0.764 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.800 | 2.155 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 36859 | |
Completeness [%] | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1M tris-HCl, 0.9M sodium citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |