4EM9

Human PPAR gamma in complex with nonanoic acids

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-07
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.46
Spacegroup name	C 1 2 1
Unit cell lengths	92.731, 62.255, 118.649
Unit cell angles	90.00, 101.94, 90.00

Refinement procedure

Resolution	32.800 - 2.100
R-factor	0.2065
Rwork	0.205
R-free	0.23510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zeo
RMSD bond length	0.005
RMSD bond angle	0.764
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.800	2.155
High resolution limit [Å]	2.100	2.100
Number of reflections	36859
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.1M tris-HCl, 0.9M sodium citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K