1ZEO

Crystal Structure of Human PPAR-gamma Ligand Binding Domain Complexed with an Alpha-Aryloxyphenylacetic Acid Agonist

Summary for 1ZEO

• Entry DOI: 10.2210/pdb1zeo/pdb
• Descriptor: Peroxisome proliferator activated receptor gamma, (2S)-(4-ISOPROPYLPHENYL)[(2-METHYL-3-OXO-5,7-DIPROPYL-2,3-DIHYDRO-1,2-BENZISOXAZOL-6-YL)OXY]ACETATE (3 entities in total)
• Functional Keywords: nuclear receptor, ligand binding domain, lbd, alpha helix sandwich, ppar-rxr heterodimer, transcription
• Biological source: Homo sapiens (human)
• Cellular location: Nucleus: P37231
• Total number of polymer chains: 2
• Total formula weight: 63611.81

Authors

Shi, G.Q.,Dropinski, J.F.,McKeever, B.M.,Adams, A.D.,MacNaul, K.L.,Elbrecht, A.,Berger, J.P.,Zhou, G.,Doebber, T.W. (deposition date: 2005-04-19, release date: 2006-04-25, Last modification date: 2024-04-03)

Primary citation

Shi, G.Q.,Dropinski, J.F.,McKeever, B.M.,Xu, S.,Becker, J.W.,Berger, J.P.,MacNaul, K.L.,Elbrecht, A.,Zhou, G.,Doebber, T.W.,Wang, P.,Chao, Y.-S.,Forrest, M.,Heck, J.V.,Moller, D.E.,Jones, B.A.
Design and Synthesis of alpha-Aryloxyphenylacetic Acid Derivatives: A Novel Class of PPAR alpha/gamma Dual Agonists with Potent Antihyperglycemic and Lipid Modulating Activity
J.Med.Chem., 48:4457-4468, 2005

PubMed Abstract: The synthesis and structure-activity relationships of novel series of alpha-aryloxyphenylacetic acids as PPARalpha/gamma dual agonists are reported. The initial search for surrogates of the ester group in the screen lead led first to the optimization of a subseries with a ketone moiety. Further efforts to modify the ketone subseries led to the design and synthesis of two new subseries containing fused heterocyclic ring systems. All these analogues were characterized by their "super" PPARalpha agonist activity and weak or partial agonist activity on PPARgamma in PPAR-GAL4 transactivation assays despite their similar binding affinities for both receptors. The cocrystal structures of compounds 7 and rosiglitazone with PPARgamma-LBD were compared, and significant differences were found in their interactions with the receptor. Select analogues in each subseries were further evaluated for in vivo efficacy. They all showed excellent anti-hyperglycemic efficacy in a db/db mouse model and hypolipidemic activity in hamster and dog models without provoking the typical PPARgamma-associated side effects in the rat tolerability assay.

PubMed: 15974597
DOI: 10.1021/jm0502135

Experimental method

X-RAY DIFFRACTION (2.5 Å)