4E4D

Crystal structure of mouse RANKL-OPG complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-01-01
Detector	NOIR-1
Wavelength(s)	0.9951
Spacegroup name	P 63
Unit cell lengths	109.763, 109.763, 78.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.700 - 2.700
R-factor	0.202
Rwork	0.194
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jtz
RMSD bond length	0.002
RMSD bond angle	0.593
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.700	5.810	2.700
Rmerge	0.084	0.029	0.563
Number of reflections	14915
<I/σ(I)>	12.8
Completeness [%]	99.3	95.6	100
Redundancy	2.9	2.9	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.1	293	100 mM sodium phosphate/citrate buffer, 14% polyethylene glycol 8000 and 250 mM sodium chloride, pH 4.10, VAPOR DIFFUSION, HANGING DROP, temperature 293K