4D8D
Crystal structure of HIV-1 NEF Fyn-SH3 R96W variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2000-04-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98064 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 108.010, 108.010, 224.961 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.513 - 2.520 |
| R-factor | 0.2135 |
| Rwork | 0.212 |
| R-free | 0.23760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1avz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.977 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 58.510 |
| High resolution limit [Å] | 2.520 |
| Number of reflections | 26791 |
| Completeness [%] | 99.1 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | 0.3 M sodium potassium tartrate, 0.5 M bicine buffer , 1cmc D-octylglucopyranoside, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
