4BKF
crystal structure of the human EphA4 ectodomain in complex with human ephrinB3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 43 3 2 |
Unit cell lengths | 300.530, 300.530, 300.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 106.250 - 4.650 |
R-factor | 0.3286 |
Rwork | 0.327 |
R-free | 0.35080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 4BK4 3d12 |
RMSD bond length | 0.010 |
RMSD bond angle | 0.990 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 106.000 | 4.700 |
High resolution limit [Å] | 4.650 | 4.650 |
Rmerge | 0.260 | |
Number of reflections | 25557 | |
<I/σ(I)> | 7.7 | 0.5 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 10.8 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | PROTEIN SAMPLE WAS MIXED 1:1:1 (V/V/V) WITH (0.08 M MAGNESIUM ACETATE TETRAHYDRATE. 0.05 M CACODYLATE PH 6.5, 15% POLYETHYLENE GLYCOL 400) AND 0.1 M SPERMIDINE (HAMPTON). | |||
1 | PROTEIN SAMPLE WAS MIXED 1:1:1 (V/V/V) WITH (0.08 M MAGNESIUM ACETATE TETRAHYDRATE. 0.05 M CACODYLATE PH 6.5, 15% POLYETHYLENE GLYCOL 400) AND 0.1 M SPERMIDINE (HAMPTON). |