4BHW
Structural basis for autoinhibition of the acetyltransferase activity of p300
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-09 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 153.051, 92.894, 123.309 |
Unit cell angles | 90.00, 98.43, 90.00 |
Refinement procedure
Resolution | 46.620 - 2.799 |
R-factor | 0.2096 |
Rwork | 0.208 |
R-free | 0.24410 |
Structure solution method | SAD |
Starting model (for MR) | 3biy |
RMSD bond length | 0.006 |
RMSD bond angle | 1.102 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((PHENIX.REFINE: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.070 | 0.590 |
Number of reflections | 80344 | |
<I/σ(I)> | 9.4 | 1.5 |
Completeness [%] | 97.4 | 97.1 |
Redundancy | 3.4 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 100 MM HEPES, PH 7.5, 20% PEG3350, 0.2M NACL |