4BDV
CRYSTAL STRUCTURE OF A TRUNCATED B-DOMAIN HUMAN FACTOR VIII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0082, 1.2700 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 133.840, 133.840, 355.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.980 |
| R-factor | 0.16626 |
| Rwork | 0.162 |
| R-free | 0.24558 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3cdz |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.096 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 4.080 |
| High resolution limit [Å] | 3.980 | 3.980 |
| Rmerge | 0.200 | 1.400 |
| Number of reflections | 28556 | |
| <I/σ(I)> | 12.45 | 2.39 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 16.6 | 17.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 3% PEG550, 0.100 M NACL, 0.100 M TRIS-HCL PH 7.5, 10% ETHANOL |
