4AZA
Improved eIF4E binding peptides by phage display guided design.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU PROTEUM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-01 |
| Detector | RIGAKU PT 135 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.662, 38.168, 121.758 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 121.760 - 2.160 |
| R-factor | 0.23526 |
| Rwork | 0.233 |
| R-free | 0.27859 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w97 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.142 |
| Data reduction software | PROTEUM2 |
| Data scaling software | PROTEUM2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.880 | 2.260 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.200 | 0.750 |
| Number of reflections | 30410 | |
| <I/σ(I)> | 5.22 | 1.38 |
| Completeness [%] | 99.3 | 94.5 |
| Redundancy | 9.22 | 6.51 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 20% PEG 5000 MME, 100 MM BISTRIS PH 6.5. |
