4AEZ

Crystal Structure of Mitotic Checkpoint Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-14
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	70.810, 286.900, 72.010
Unit cell angles	90.00, 119.04, 90.00

Refinement procedure

Resolution	62.957 - 2.300
R-factor	0.2215
Rwork	0.221
R-free	0.26710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2VFX 2wvi 3emh
RMSD bond length	0.006
RMSD bond angle	0.959
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.960	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.060	0.590
Number of reflections	110660
<I/σ(I)>	16.4	3.1
Completeness [%]	99.9	100
Redundancy	5.5	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8		CRYSTALS WERE OBTAINED IN A HANGING-DROP FASHION BY PRE-INCUBATING 1:1 V/V OF 4.5 MG/ML OF PROTEIN WITH THE CRYSTALLIZATION SOLUTION, 100 MM TRIS-HCL PH 8.8, 21% PEG 3350, 30% ETHYLENE GLYCOL, 5 MM DTT, AND 5 MM EDTA AT 20 OC FOR 24 HOURS, FOLLOWED BY STREAK SEEDING.