3ZGZ
Ternary complex of E. coli leucyl-tRNA synthetase, tRNA(leu) and toxic moiety from agrocin 84 (TM84) in aminoacylation-like conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-27 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.580, 68.190, 226.220 |
| Unit cell angles | 90.00, 105.53, 90.00 |
Refinement procedure
| Resolution | 43.590 - 2.400 |
| R-factor | 0.18941 |
| Rwork | 0.187 |
| R-free | 0.23822 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4aq7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.449 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.100 | 0.440 |
| Number of reflections | 85255 | |
| <I/σ(I)> | 10.2 | 1.8 |
| Completeness [%] | 93.3 | 65.1 |
| Redundancy | 3.91 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.1 M BIS-TRIS, 23-25 % PEG 3350, PH 5.5 |
