3ZGZ

Ternary complex of E. coli leucyl-tRNA synthetase, tRNA(leu) and toxic moiety from agrocin 84 (TM84) in aminoacylation-like conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, D)	LEUCINE--TRNA LIGASE	polymer	880	99516.0	2	UniProt (P07813) Pfam (PF09334) Pfam (PF13603) Pfam (PF00133) Pfam (PF08264)	ESCHERICHIA COLI	LEUCYL-TRNA SYNTHETASE, LEURS
2	B, D (B, E)	TRNA-LEU UAA ISOACCEPTOR	polymer	88	28396.9	2		ESCHERICHIA COLI K-12
3	E, L (A, D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	F, M (A, D)	[(2S,4S,5R)-5-(6-aminopurin-9-yl)-4-oxidanyl-oxolan-2-yl]methoxy-N-[(2S,3R)-4-methyl-2,3-bis(oxidanyl)pentanoyl]phosphonamidic acid	non-polymer		460.4	2	Chemie (84T)
5	G, H, I, J, K... (B, E)	MAGNESIUM ION	non-polymer		24.3	7	Chemie (MG)
6	P, Q, R, S (A, B, D, E)	water	water		18.0	369	Chemie (HOH)

Sequence modifications

A, D: 1 - 860 (UniProt: P07813)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	255825.8
Non-Polymers*	Number of molecules	11
	Total formula weight	1221.7
All*	Total formula weight	257047.5

*Water molecules are not included.