3WLC
Crystal structure of dimeric GCaMP6m
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-08-15 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.440, 46.860, 68.340 |
| Unit cell angles | 90.00, 99.36, 90.00 |
Refinement procedure
| Resolution | 20.079 - 2.490 |
| R-factor | 0.1794 |
| Rwork | 0.177 |
| R-free | 0.22480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sg4 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.871 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.080 | 2.620 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.078 | 0.251 |
| Number of reflections | 13660 | |
| <I/σ(I)> | 12.3 | 4.6 |
| Completeness [%] | 98.6 | 91.4 |
| Redundancy | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M HEPES, 20% w/v PEG 3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
