3WLC

Crystal structure of dimeric GCaMP6m

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Myosin light chain kinase, Green fluorescent protein, Calmodulin	polymer	450	50398.2	1	UniProt (Q6LDG3) UniProt (P42212) UniProt (P0DP29) Pfam (PF13499) UniProt (by SIFTS) (P0DP31)	Gallus gallus (chicken)	CaM
2	B, C, D, E (A)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
3	F (A)	water	water		18.0	159	Chemie (HOH)

Sequence modifications

A: 40 - 58 (UniProt: Q6LDG3)

PDB	External Database	Details
Met 1	-	expression tag
Gly 2	-	expression tag
Ser 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
Gly 10	-	expression tag
Met 11	-	expression tag
Ala 12	-	expression tag
Ser 13	-	expression tag
Met 14	-	expression tag
Thr 15	-	expression tag
Gly 16	-	expression tag
Gly 17	-	expression tag
Gln 18	-	expression tag
Gln 19	-	expression tag
Met 20	-	expression tag
Gly 21	-	expression tag
Arg 22	-	expression tag
Asp 23	-	expression tag
Leu 24	-	expression tag
Tyr 25	-	expression tag
Asp 26	-	expression tag
Asp 27	-	expression tag
Asp 28	-	expression tag
Asp 29	-	expression tag
Lys 30	-	expression tag
Asp 31	-	expression tag
Leu 32	-	expression tag
Ala 33	-	expression tag
Thr 34	-	expression tag
Met 35	-	expression tag
Val 36	-	expression tag
Asp 37	-	expression tag
Ser 38	-	expression tag
Ser 39	-	expression tag
Asn 44	Gln 41	engineered mutation
Leu 59	-	linker
Glu 60	-	linker

A: 61 - 150 (UniProt: P42212)

PDB	External Database	Details
Lys 65	Met 153	engineered mutation
Ala 75	Val 163	engineered mutation
Gly 87	Ser 175	engineered mutation
Tyr 92	Asp 180	engineered mutation
Val 115	Thr 203	engineered mutation
Lys 118	Ala 206	engineered mutation
Leu 143	His 231	engineered mutation

A: 159 - 301 (UniProt: P42212)

PDB	External Database	Details
Gly 151	-	linker
Gly 152	-	linker
Thr 153	-	linker
Gly 154	-	linker
Gly 155	-	linker
Ser 156	-	linker
Met 157	-	linker
Val 158	-	linker
Leu 221	Phe 64	engineered mutation
Cro 223	Ser 65	chromophore
Cro 223	Tyr 66	chromophore
Cro 223	Gly 67	chromophore
Ile 250	Val 93	engineered mutation
Leu 302	-	linker
Pro 303	-	linker

A: 304 - 450 (UniProt: P0DP29)

PDB	External Database	Details
Asp 362	Asn 61	engineered mutation
Gly 378	Met 77	engineered mutation
Ser 379	Lys 78	engineered mutation
Tyr 380	Asp 79	engineered mutation
Arg 381	Thr 80	engineered mutation
Thr 383	Ser 82	engineered mutation
Gly 392	Arg 91	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	50398.2
Non-Polymers*	Number of molecules	4
	Total formula weight	160.3
All*	Total formula weight	50558.5

*Water molecules are not included.