Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3WLC

Crystal structure of dimeric GCaMP6m

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005509	molecular_function	calcium ion binding
A	0006091	biological_process	generation of precursor metabolites and energy
A	0008218	biological_process	bioluminescence

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 501

Chain	Residue
A	ASP395
A	ASP397
A	ASN399
A	TYR401
A	GLU406
A	HOH623

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 502

Chain	Residue
A	GLN437
A	GLU442
A	HOH613
A	ASP431
A	ASP433
A	ASP435

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA A 503

Chain	Residue
A	ASP358
A	ASP360
A	ASP362
A	THR364
A	GLU369

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 504

Chain	Residue
A	ASP322
A	ASP324
A	ASP326
A	THR328
A	GLU333
A	HOH617

Functional Information from PROSITE/UniProt

site_id	PS00018
Number of Residues	13
Details	EF_HAND_1 EF-hand calcium-binding domain. DKDGDGTITtkEL

Chain	Residue	Details
A	ASP324-THR336
A	ASP360-THR372
A	ASP397-HIS409
A	ASP433-GLN445

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	35
Details	Domain: {"description":"EF-hand 1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00448","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	35
Details	Domain: {"description":"EF-hand 2","evidences":[{"source":"PROSITE-ProRule","id":"PRU00448","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	35
Details	Domain: {"description":"EF-hand 3","evidences":[{"source":"PROSITE-ProRule","id":"PRU00448","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	5
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00448","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"11323678","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23109337","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"3145979","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1G4Y","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1NIW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2HQW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2YGG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3B32","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3BXK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3BXL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3CLN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3IFK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3SJQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4EHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4G27","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4G28","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4I2Y","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4J9Y","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4J9Z","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4QNH","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	5
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00448","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"11323678","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23109337","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"3145979","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1G4Y","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1NIW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2HQW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2YGG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3B32","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3BXK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3BXL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3CLN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3EK4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3EK7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3EK8","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3EKH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3EVR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3EVU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3IFK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3SG2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3SG3","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3SG4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3SG5","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3SG6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3SG7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3SJQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3WLC","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3WLD","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4EHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4G27","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4G28","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4I2Y","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4J9Y","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4J9Z","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4QNH","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	5
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00448","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"23109337","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"3145979","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1NIW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2HQW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2YGG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3BXK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3BXL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3CLN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3EK4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3EK7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3EK8","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3EKH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3EVR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3EVU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3SG2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3SG3","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3SG4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3SG5","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3SG6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3SG7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3SJQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3WLC","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3WLD","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4EHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4I2Y","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4RJD","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	5
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00448","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"23109337","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"3145979","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1NIW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2HQW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2YGG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3BXK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3BXL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3CLN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3EK7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3EK8","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3EKH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3EVR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3EVU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3SG2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3SG3","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3SG4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3SG5","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3SG7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3SJQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3WLC","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3WLD","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4EHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4I2Y","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4RJD","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	1
Details	Modified residue: {"description":"N-acetylalanine","evidences":[{"source":"PubMed","id":"201628","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"FEB-2007","submissionDatabase":"UniProtKB","authors":["Lubec G.","Chen W.-Q."]}}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	1
Details	Modified residue: {"description":"N6-acetyllysine; alternate","evidences":[{"source":"UniProtKB","id":"P0DP23","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	1
Details	Modified residue: {"description":"Phosphothreonine; by CaMK4","evidences":[{"source":"PubMed","id":"12392717","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P0DP23","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	1
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P0DP23","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI13
Number of Residues	1
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"PubMed","id":"22673903","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI14
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"22673903","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI15
Number of Residues	1
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"UniProtKB","id":"P0DP23","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI16
Number of Residues	1
Details	Modified residue: {"description":"N6-methyllysine; alternate","evidences":[{"source":"UniProtKB","id":"P0DP23","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI17
Number of Residues	1
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"P0DP23","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI18
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate","evidences":[{"source":"UniProtKB","id":"P62157","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI19
Number of Residues	19
Details	Region: {"description":"Calmodulin recognition (RS20) region","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI20
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine; by PKG","evidences":[{"source":"PubMed","id":"12176732","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI21
Number of Residues	1
Details	Modified residue: {"description":"(Z)-2,3-didehydrotyrosine","evidences":[{"source":"PubMed","id":"8448132","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI22
Number of Residues	2
Details	Cross-link: {"description":"5-imidazolinone (Ser-Gly)","evidences":[{"source":"PubMed","id":"8448132","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)