3SG4

Crystal Structure of GCaMP3-D380Y, LP(linker 2)

Summary for 3SG4

Related3SG2 3SG3 3SG5 3SG6 3SG7
DescriptorMyosin light chain kinase, Green fluorescent protein, Calmodulin-1 chimera, CALCIUM ION (3 entities in total)
Functional Keywordscalcium sensor, fluorescent protein
Biological sourceGallus gallus (Chicken)
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Cellular locationCytoplasm, cytoskeleton, spindle P0DP29
Total number of polymer chains1
Total molecular weight50704.76
Authors
Schreiter, E.R.,Akerboom, J.,Looger, L.L. (deposition date: 2011-06-14, release date: 2012-06-20, Last modification date: 2017-06-28)
Primary citation
Akerboom, J.,Chen, T.W.,Wardill, T.J.,Tian, L.,Marvin, J.S.,Mutlu, S.,Calderon, N.C.,Esposti, F.,Borghuis, B.G.,Sun, X.R.,Gordus, A.,Orger, M.B.,Portugues, R.,Engert, F.,Macklin, J.J.,Filosa, A.,Aggarwal, A.,Kerr, R.A.,Takagi, R.,Kracun, S.,Shigetomi, E.,Khakh, B.S.,Baier, H.,Lagnado, L.,Wang, S.S.,Bargmann, C.I.,Kimmel, B.E.,Jayaraman, V.,Svoboda, K.,Kim, D.S.,Schreiter, E.R.,Looger, L.L.
Optimization of a GCaMP calcium indicator for neural activity imaging.
J.Neurosci., 32:13819-13840, 2012
PubMed: 23035093 (PDB entries with the same primary citation)
DOI: 10.1523/JNEUROSCI.2601-12.2012
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.4 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.21030.8%3.5%6.3%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 3sg4
no rotation
Molmil generated image of 3sg4
rotated about x axis by 90°
Molmil generated image of 3sg4
rotated about y axis by 90°