3SG4
Crystal Structure of GCaMP3-D380Y, LP(linker 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-04 |
| Detector | RAYONIX MX225HE |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 120.184, 120.184, 97.352 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.660 - 2.400 |
| R-factor | 0.17411 |
| Rwork | 0.172 |
| R-free | 0.21271 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ek8 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.913 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0111) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.700 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 26965 | |
| <I/σ(I)> | 14.7 | 3.7 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 13.7 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2 M sodium chloride, 0.1 M Tris, pH 8.5, 25% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
