3VP4

Crystal structure of human glutaminase in complex with inhibitor 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Detector technology	CCD
Collection date	2010-12-20
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	I 41 2 2
Unit cell lengths	139.555, 139.555, 156.866
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.450
Rwork	0.198
R-free	0.21620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3czd
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.450
Number of reflections	28767
Completeness [%]	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.65M Lithium sulfate, 100mM Bis-tris-propane pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K