3VJO
Crystal structure of the wild-type EGFR kinase domain in complex with AMPPNP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2007-08-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 143.360, 143.360, 143.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.340 - 2.640 |
| Rwork | 0.190 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gs2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.800 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.610 | 2.740 |
| High resolution limit [Å] | 2.640 | 2.640 |
| Number of reflections | 14516 | |
| <I/σ(I)> | 30.2 | 6.47 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 6.669 | 6.905 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M Tris-HCl(pH 7.0), 0.2M NaCl, 1M Sodium citrate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
