3V9W
Crystal structure of RNase T in complex with a preferred ssDNA (TTA) with two Mg in the active site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9999 |
Spacegroup name | P 32 |
Unit cell lengths | 46.340, 46.340, 315.354 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.394 - 1.702 |
R-factor | 0.1944 |
Rwork | 0.192 |
R-free | 0.22620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ngy |
RMSD bond length | 0.004 |
RMSD bond angle | 0.872 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 80910 | |
<I/σ(I)> | 23.91 | 3.03 |
Completeness [%] | 97.3 | 99.7 |
Redundancy | 2.6 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 6%(v/v) Tacsimate, 0.1M MES monohydrate, 25%(w/v) Polyethylene glycol 4000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |