3V9W

Crystal structure of RNase T in complex with a preferred ssDNA (TTA) with two Mg in the active site

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000175	molecular_function	3'-5'-RNA exonuclease activity
A	0000287	molecular_function	magnesium ion binding
A	0003676	molecular_function	nucleic acid binding
A	0004518	molecular_function	nuclease activity
A	0004527	molecular_function	exonuclease activity
A	0004540	molecular_function	RNA nuclease activity
A	0005515	molecular_function	protein binding
A	0005829	cellular_component	cytosol
A	0006259	biological_process	DNA metabolic process
A	0006396	biological_process	RNA processing
A	0006974	biological_process	DNA damage response
A	0008033	biological_process	tRNA processing
A	0008310	molecular_function	single-stranded DNA 3'-5' DNA exonuclease activity
A	0008408	molecular_function	3'-5' exonuclease activity
A	0016787	molecular_function	hydrolase activity
A	0016896	molecular_function	RNA exonuclease activity, producing 5'-phosphomonoesters
A	0031125	biological_process	rRNA 3'-end processing
A	0034644	biological_process	cellular response to UV
A	0042780	biological_process	tRNA 3'-end processing
A	0042802	molecular_function	identical protein binding
A	0042803	molecular_function	protein homodimerization activity
A	0043628	biological_process	regulatory ncRNA 3'-end processing
A	0045004	biological_process	DNA replication proofreading
A	0046872	molecular_function	metal ion binding
B	0000175	molecular_function	3'-5'-RNA exonuclease activity
B	0000287	molecular_function	magnesium ion binding
B	0003676	molecular_function	nucleic acid binding
B	0004518	molecular_function	nuclease activity
B	0004527	molecular_function	exonuclease activity
B	0004540	molecular_function	RNA nuclease activity
B	0005515	molecular_function	protein binding
B	0005829	cellular_component	cytosol
B	0006259	biological_process	DNA metabolic process
B	0006396	biological_process	RNA processing
B	0006974	biological_process	DNA damage response
B	0008033	biological_process	tRNA processing
B	0008310	molecular_function	single-stranded DNA 3'-5' DNA exonuclease activity
B	0008408	molecular_function	3'-5' exonuclease activity
B	0016787	molecular_function	hydrolase activity
B	0016896	molecular_function	RNA exonuclease activity, producing 5'-phosphomonoesters
B	0031125	biological_process	rRNA 3'-end processing
B	0034644	biological_process	cellular response to UV
B	0042780	biological_process	tRNA 3'-end processing
B	0042802	molecular_function	identical protein binding
B	0042803	molecular_function	protein homodimerization activity
B	0043628	biological_process	regulatory ncRNA 3'-end processing
B	0045004	biological_process	DNA replication proofreading
B	0046872	molecular_function	metal ion binding
C	0000175	molecular_function	3'-5'-RNA exonuclease activity
C	0000287	molecular_function	magnesium ion binding
C	0003676	molecular_function	nucleic acid binding
C	0004518	molecular_function	nuclease activity
C	0004527	molecular_function	exonuclease activity
C	0004540	molecular_function	RNA nuclease activity
C	0005515	molecular_function	protein binding
C	0005829	cellular_component	cytosol
C	0006259	biological_process	DNA metabolic process
C	0006396	biological_process	RNA processing
C	0006974	biological_process	DNA damage response
C	0008033	biological_process	tRNA processing
C	0008310	molecular_function	single-stranded DNA 3'-5' DNA exonuclease activity
C	0008408	molecular_function	3'-5' exonuclease activity
C	0016787	molecular_function	hydrolase activity
C	0016896	molecular_function	RNA exonuclease activity, producing 5'-phosphomonoesters
C	0031125	biological_process	rRNA 3'-end processing
C	0034644	biological_process	cellular response to UV
C	0042780	biological_process	tRNA 3'-end processing
C	0042802	molecular_function	identical protein binding
C	0042803	molecular_function	protein homodimerization activity
C	0043628	biological_process	regulatory ncRNA 3'-end processing
C	0045004	biological_process	DNA replication proofreading
C	0046872	molecular_function	metal ion binding
D	0000175	molecular_function	3'-5'-RNA exonuclease activity
D	0000287	molecular_function	magnesium ion binding
D	0003676	molecular_function	nucleic acid binding
D	0004518	molecular_function	nuclease activity
D	0004527	molecular_function	exonuclease activity
D	0004540	molecular_function	RNA nuclease activity
D	0005515	molecular_function	protein binding
D	0005829	cellular_component	cytosol
D	0006259	biological_process	DNA metabolic process
D	0006396	biological_process	RNA processing
D	0006974	biological_process	DNA damage response
D	0008033	biological_process	tRNA processing
D	0008310	molecular_function	single-stranded DNA 3'-5' DNA exonuclease activity
D	0008408	molecular_function	3'-5' exonuclease activity
D	0016787	molecular_function	hydrolase activity
D	0016896	molecular_function	RNA exonuclease activity, producing 5'-phosphomonoesters
D	0031125	biological_process	rRNA 3'-end processing
D	0034644	biological_process	cellular response to UV
D	0042780	biological_process	tRNA 3'-end processing
D	0042802	molecular_function	identical protein binding
D	0042803	molecular_function	protein homodimerization activity
D	0043628	biological_process	regulatory ncRNA 3'-end processing
D	0045004	biological_process	DNA replication proofreading
D	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG A 301

Chain	Residue
A	ASP23
A	GLU25
A	HIS181
A	ASP186
E	DA7

---

site_id	AC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CO A 302

Chain	Residue
A	ARG135
A	HOH423
A	HOH429

---

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG B 301

Chain	Residue
B	GLU25
B	HIS181
B	ASP186
F	DA7
B	ASP23

---

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CO B 302

Chain	Residue
B	ARG135
B	HOH429
B	HOH451

---

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CO C 301

Chain	Residue
C	GLU25
C	HIS181
C	ASP186
G	DA7

---

site_id	AC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CO C 302

Chain	Residue
C	GLY92
C	ARG135
C	HOH571

---

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CO D 301

Chain	Residue
D	GLU25
D	HIS181
D	ASP186
H	DA7

---

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CO D 302

Chain	Residue
D	ARG135
D	HOH415
D	HOH420
D	HOH441

---

site_id	AC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG E 101

Chain	Residue
A	ASP23
A	HOH403
A	HOH404
A	HOH434
E	DT6
E	DA7

---

site_id	BC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG F 101

Chain	Residue
B	ASP23
B	HOH403
B	HOH409
B	HOH434
F	DT6
F	DA7

---

site_id	BC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG G 101

Chain	Residue
C	ASP23
C	HOH403
C	HOH406
C	HOH409
G	DT6
G	DA7

---

site_id	BC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG H 101

Chain	Residue
D	ASP23
D	HOH403
D	HOH405
D	HOH411
H	DT6
H	DA7

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	696
Details	Domain: {"description":"Exonuclease","evidences":[{"source":"HAMAP-Rule","id":"MF_00157","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	Active site: {"description":"Proton donor/acceptor","evidences":[{"source":"HAMAP-Rule","id":"MF_00157","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"21317904","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"22718982","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	16
Details	Binding site: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	20
Details	Site: {"description":"Important for substrate binding and specificity"}

Chain

Residue

Details

---