3V9W
Crystal structure of RNase T in complex with a preferred ssDNA (TTA) with two Mg in the active site
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Ribonuclease T | polymer | 235 | 25647.0 | 4 | UniProt (P30014) Pfam (PF00929) In PDB | Escherichia coli | Exoribonuclease T, RNase T |
2 | E, F, G, H | DNA (5'-D(*GP*CP*TP*TP*A)-3') | polymer | 5 | 1495.0 | 4 | |||
3 | A, B, E, F, G... | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) | |||
4 | A, B, C, D | COBALT (II) ION | non-polymer | 58.9 | 6 | Chemie (CO) | |||
5 | water | water | 18.0 | 804 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 215 (UniProt: P30014)
PDB | External Database | Details |
---|---|---|
Met -19 | - | expression tag |
Gly -18 | - | expression tag |
Ser -17 | - | expression tag |
Ser -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
Leu -6 | - | expression tag |
Val -5 | - | expression tag |
Pro -4 | - | expression tag |
Arg -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
Gly 92 | Glu 92 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 8 |
Total formula weight | 108568.0 | |
Non-Polymers* | Number of molecules | 12 |
Total formula weight | 499.4 | |
All* | Total formula weight | 109067.4 |