3UZS
Structure of the C13.28 RNA Aptamer Bound to the G Protein-Coupled Receptor Kinase 2-Heterotrimeric G Protein Beta 1 and Gamma 2 Subunit Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2008-07-01 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97856 |
Spacegroup name | P 3 1 2 |
Unit cell lengths | 257.277, 257.277, 99.429 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.990 - 4.520 |
R-factor | 0.18758 |
Rwork | 0.184 |
R-free | 0.26837 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1omw |
RMSD bond length | 0.005 |
RMSD bond angle | 0.856 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.580 |
High resolution limit [Å] | 4.500 | 4.500 |
Number of reflections | 12758 | |
<I/σ(I)> | 24.5 | 1.6 |
Completeness [%] | 58.4 | 9 |
Redundancy | 3.9 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 100 mM Tris pH 8.5, 200 mM NaCl and 3% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |