3UZS

Structure of the C13.28 RNA Aptamer Bound to the G Protein-Coupled Receptor Kinase 2-Heterotrimeric G Protein Beta 1 and Gamma 2 Subunit Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Beta-adrenergic receptor kinase 1	polymer	689	79749.9	1	UniProt (P21146) Pfam (PF00615) Pfam (PF00069) Pfam (PF00169)	Bos taurus (bovine)	Beta-ARK-1, G-protein-coupled receptor kinase 2
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62871) Pfam (PF00400)	Bos taurus (bovine)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	74	8406.7	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (bovine)	G gamma-I
4	D (C)	C13.28 RNA Aptamer	polymer	28	9079.5	1
5	E (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

A: 1 - 681 (UniProt: P21146)

PDB	External Database	Details
Ala 670	Ser 670	engineered mutation

G: 1 - 67 (UniProt: P63212)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Cmt 68	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	134653.0
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	134677.3

*Water molecules are not included.