3ULU
Structure of quaternary complex of human TLR3ecd with three Fabs (Form1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-03 |
Detector | RIGAKU SATURN 944 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 214.900, 142.080, 125.040 |
Unit cell angles | 90.00, 103.17, 90.00 |
Refinement procedure
Resolution | 45.075 - 3.520 |
R-factor | 0.2701 |
Rwork | 0.268 |
R-free | 0.30910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 2A0Z 1ziw 3uls 3na9 3qpq |
RMSD bond length | 0.002 |
RMSD bond angle | 0.611 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_896)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.700 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.202 | 0.682 |
Number of reflections | 34547 | |
<I/σ(I)> | 8.4 | 2.2 |
Completeness [%] | 74.1 | 12.6 |
Redundancy | 5.3 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.5 | 293 | 0.1 M sodium acetate, pH 4.5, 28% PEG3350, 1 M lithium chloride, 30 mM Glycyl-glycyl-glycine, VAPOR DIFFUSION, SITTING DROP, temperature 293K |