3U0Z
Tetramerization dynamics of the C-terminus underlies isoform-specific cAMP-gating in HCN channels
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97238 |
| Spacegroup name | I 4 |
| Unit cell lengths | 97.670, 97.670, 113.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.780 - 2.900 |
| R-factor | 0.20812 |
| Rwork | 0.205 |
| R-free | 0.27497 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q43 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.708 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.780 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.120 | 0.485 |
| Number of reflections | 11821 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 278 | 20-22% PEG 3350, 0.4M Sodium Acetate buffer (pH 5.0), VAPOR DIFFUSION, temperature 278K |
