3SWC
GLP (G9a-like protein) SET domain in complex with Dnmt3aK44me2 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.090, 90.220, 95.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.780 - 2.332 |
R-factor | 0.1897 |
Rwork | 0.187 |
R-free | 0.22740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB 3MO5 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.137 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.780 | 2.400 |
High resolution limit [Å] | 2.330 | 2.330 |
Rmerge | 0.072 | 0.406 |
Number of reflections | 29318 | |
<I/σ(I)> | 26 | 5.6 |
Completeness [%] | 98.1 | 95.9 |
Redundancy | 8.8 | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1 M Hepes, 16% polyethylene glycol 4000 and 8% isopropanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |