3SIP
Crystal structure of drICE and dIAP1-BIR1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.25627 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 91.350, 148.115, 156.738 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.091 - 3.496 |
| R-factor | 0.2122 |
| Rwork | 0.210 |
| R-free | 0.25560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i3o |
| RMSD bond length | 0.042 |
| RMSD bond angle | 1.405 |
| Refinement software | PHENIX ((phenix.refine: 1.6.3_473)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.496 |
| Number of reflections | 13767 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.1M Hepes pH7.0, 0.3M(NH4)2SO4, 20% (wt/vol) Polyethylene Glycol 3350 , VAPOR DIFFUSION, HANGING DROP, temperature 291K |
