3SDJ
Structure of RNase-inactive point mutant of oligomeric kinase/RNase Ire1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2009-11-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.115879 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 158.790, 163.450, 298.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.652 - 3.650 |
R-factor | 0.2505 |
Rwork | 0.248 |
R-free | 0.28730 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.027 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.652 |
High resolution limit [Å] | 3.650 |
Number of reflections | 86573 |
Completeness [%] | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.6 | 300 | SODIUM CITRATE, pH 5.6, VAPOR DIFFUSION, temperature 300K |