3S9D
binary complex between IFNa2 and IFNAR2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-05 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 84.650, 62.010, 88.080 |
Unit cell angles | 90.00, 92.68, 90.00 |
Refinement procedure
Resolution | 19.897 - 2.000 |
Rwork | 0.204 |
R-free | 0.23300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 3S8W 2hym and 1RH2 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.140 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.897 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 61371 | |
<I/σ(I)> | 17.6 | 2.6 |
Completeness [%] | 99.1 | 99.1 |
Redundancy | 3.7 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 293 | 20% (w/v) PEG 3350, 200 mM NaSCN, VAPOR DIFFUSION, HANGING DROP, temperature 293K |