2HYM

NMR based Docking Model of the Complex between the Human Type I Interferon Receptor and Human Interferon alpha-2

Summary for 2HYM

• Entry DOI: 10.2210/pdb2hym/pdb
• Related: 1ITF 1N6V
• Descriptor: Soluble IFN alpha/beta receptor, Interferon alpha-2 (2 entities in total)
• Functional Keywords: interferon receptor complex, immune system
• Biological source: Homo sapiens (human); More
• Cellular location: Secreted: P01563
• Total number of polymer chains: 2
• Total formula weight: 43587.53

Authors

Quadt-Akabayov, S.R.,Chill, J.H.,Levy, R.,Kessler, N.,Anglister, J. (deposition date: 2006-08-07, release date: 2006-10-10, Last modification date: 2024-10-16)

Primary citation

Quadt-Akabayov, S.R.,Chill, J.H.,Levy, R.,Kessler, N.,Anglister, J.
Determination of the human type I interferon receptor binding site on human interferon-alpha2 by cross saturation and an NMR-based model of the complex
Protein Sci., 15:2656-2668, 2006

PubMed Abstract: Type I interferons (IFNs) are a family of homologous helical cytokines that exhibit pleiotropic effects on a wide variety of cell types, including antiviral activity and antibacterial, antiprozoal, immunomodulatory, and cell growth regulatory functions. Consequently, IFNs are the human proteins most widely used in the treatment of several kinds of cancer, hepatitis C, and multiple sclerosis. All type I IFNs bind to a cell surface receptor consisting of two subunits, IFNAR1 and IFNAR2, associating upon binding of interferon. The structure of the extracellular domain of IFNAR2 (R2-EC) was solved recently. Here we study the complex and the binding interface of IFNalpha2 with R2-EC using multidimensional NMR techniques. NMR shows that IFNalpha2 does not undergo significant structural changes upon binding to its receptor, suggesting a lock-and-key mechanism for binding. Cross saturation experiments were used to determine the receptor binding site upon IFNalpha2. The NMR data and previously published mutagenesis data were used to derive a docking model of the complex with an RMSD of 1 Angstrom, and its well-defined orientation between IFNalpha2 and R2-EC and the structural quality greatly improve upon previously suggested models. The relative ligand-receptor orientation is believed to be important for interferon signaling and possibly one of the parameters that distinguish the different IFN I subtypes. This structural information provides important insight into interferon signaling processes and may allow improvement in the development of therapeutically used IFNs and IFN-like molecules.

PubMed: 17001036
DOI: 10.1110/ps.062283006

Experimental method

SOLUTION NMR